Transgenic Mouse Lines

The following tables comprise mouse lines expressing the Tet transactivators (tTA [Gossen and Bujard, 1992] rtTA [Gossen et al., 1995] rtTA2 S-M2 or rtTA2 S-S2 [Urlinger et al., 2000]) under control of various promoters (Table 1) and responsive mouse lines carrying target genes under Tet-control (Table 2) .

These lists may not be complete as the “pool” of Tet mice is continuously increasing. Therefore if you are aware of additional mouse lines that should be included, please contact us . Tables will be updated on a regular basis and are a service that we provide to the research community.

Scientists receiving requests for Tet mice please find the ‘Notice and Acknowledgement Agreement’ (N&A) that has to be completed for the benefit of TET Systems Holding before transfer of any Tet mice. Please click on N&A 1 if the requesting institution is governed by the Federal Tort Claims Act (FTCA 28 U.S.C.). All other institutions please use N&A 2.

Table 1: Mouse lines expressing tTA or rtTA genes

Promoter (origin)	Tissue specificity	tTA	rtTA	References
Albumin / MUP (mouse urinary protein promoter)	Liver	+		Manickan E et al (2001)
astrocyte-specific glial fibrillary acidic protein	neuronal cells		+	Kim BO et al (2003)
Axin2 (mouse)	developing kidney (Wolfian duct and ureteric bud epithelia)		rtTA²S-M2	Shakya R. et al. 2005
bovine keratin 5 (K5)	epithelial cells		+	Vitale-Cross et al (2004)
αCaMKII ¹⁾	brain	+		Mayford M et al (1996)
αCaMKII	brain		+	Mansuy IM et al (1998)
CD2	T cell lineage		+	Legname G et al (2000)
CD34	early bone marrow progentor and stem cells	+		Radomska HS et al (2002)
ckit	retinal ganglion cells		rtTA²S-M2	Kerrison JB et al. 2005
clara cell 10 kd protein (CC10)	lung airway (parenchyma)		+	Mehrad B et al (2002)
Clara cell secretory protein (CCSP)	respiratory epithelial cells		+	Tichelaar JW et al (2000)
hCMV	various tissues	+	+	Kistner A et al (1996)
E α enhancer and Ii	lung, spleen, thymus		rtTA2^S-S2	Obst R et al. 2005
endothelin receptor B (EDNRB)	melanocytes, neural crest	+	+	Shin MK et al (1999)
Fatty acid binding protein (Fbp)	small intestine, cecum, colon, bladder		+	Saam JR et al (1999)
GFAP	CNS - astrocytes	+		Lin W. et al. 2004
Growth hormone (GH)	pituitary gland		+	Roh M et al (2001)
hGFAP (glial fibrillary acidic protein)	astrocytes	+		Pascual O et al. 2005
Hoxb7 (mouse)	developing kidney (Wolfian duct and ureteric bud epithelia)		rtTA²S-M2	Shakya R. et al. 2005
Intronic IgH enhancer, minimal promoter	thymus, bone marrow	+		Hess J et al (2001)
Immunoglobulin heavy chain enhancer and SR α promoter	hematopoietic system	+		Felsher DW et al (1999)
Insulin	pancreatic β - cells	+		Efrat S et al (1995)
Insulin	pancreatic β - cells		+	Thomas MK et al (2001)
Interphotoreceptor retinoid-binding protein (IRBP)	Photoreceptor cells		+	Chang MA et al (2000)
Kerapr(mouse)	cornea keratocytes		+	Hayashi M. et al. 2005
keratin 5 (K5)	epidermis, hair follicle	+	+	Diamond I et al (2000)
keratin 6 (K6)	keratinocytes	+		Guo Y et al (1999)
keratin 14 (K14)	epidermis, squamous epithelia		+	Xie W et al (1999)
keratin 14 (K14)	mammary gland	+		Dunbar ME et al (2001)
keratin 18 (K18)	trachea, upper bronchi, submucosal glands		+	Ye L et al (2001)
Krt12-Knock-in (mouse)	corneal epithelium		IRES-rtTA	Chikama T. et al. 2005
Lck	T cell lineage	+		Leenders H et al (2000)
liver enriched activator protein (LAP) ¹⁾	liver	+		Kistner A et al (1996)
liver enriched activator protein (LAP)	liver		+	Schönig K et al (2002)
major histocompatibility complex (MHC) class II E α	thymic, epithelial, dentritic, B cells, macrophages,	+		Witherden D et al (2000)
α–myosin heavy chain (MHC α) ¹⁾	heart muscle	+		Passman RS et al (1994)
α–myosin heavy chain (MHC α)	heart muscle		+	Valencik ML et al (2001)
major urinary protein (MUP)	liver	+		Manickan E et al (2001)
MLC1 (rat)	skeletal muscle		rtTA²S-M2	Ponomareva O. et al. 2005
MMTV long terminal repeat ¹⁾	mammary gland, salivery gland, seminal vesicle	+		Hennighausen L et al (1995)
MMTV long terminal repeat	mammary gland, salivery gland, seminal vesicle		+	D´Cruz CM et al (2001)
muscle creatine kinase (MCK)	muscle	+		Ghersa P et al (1998)
nestin	various tissues		+	Mitsuhashi T et al (2001)
nestin	various tissues		rtTA²S-M2	Yu TS et al. 2005
Neuron -specific enolase (NSE) ¹⁾	brain	+		Chen J et al (1998)
P0	Schwann cells		+	Pot C et al (2002)
P2	olfactory sensory neurons		+	Gogos JA et al (2000)
pdx-1	pancreas	+		Holland AM et al (2002)
Peripheral myelin protein 22 (PMP22)	Schwann cells	+		Perea J et al (2001)
PF4 (rat)	megakaryocytes / platelets	+		Nguyen H. et al. 2005
PrP	brain	+		Tremblay P et al (1998)
prolactin	pituitary gland	+		Roh M et al (2001)
Retinoblastoma gene (Rb)	brain, lung, spleen	+		Nikitin AY et al (2001)
rhodopsin	photoreceptor cells		+	Chang MA et al (2000)
ROSA 26	various tissues depending on CRE expression		loxP-STOP-loxP rtTA²S-M2	Yu HM et al. 2005
Stem cell leukemia (SCL) gene 3’ enhancer	hematopoietic cells	+		Koschmieder S et al (2005)
SM 22 (mouse)	smooth muscle cells		+	West J. et al. 2004
SM22 α	smooth muscle cells	+		Ju H et al (2001)
surfactant protein-C (SP/C)	respiratory epithelial cells		+	Tichelaar JW et al (2000)
Tek	endothelial cells	+		Sarao R et al (1998)
Thy1	retinal ganglion cells		rtTA²S-M2	Kerrison JB et al. 2005
Tie	endothelial cells	+		Sarao R et al (1998)
Tie2	vascular endothelium		+	Teng PI et al (2002)
tyrosinase	melanocytes		+	Chin L et al (1999)
tyrosinase LCR (mouse)	retina		+	Gimenez E et al (2004)
Vav	hematopoietic cells	+		Wiesner SM et al (2004)
Vav	hemotopoietic system	+		Wiesner SM et al. 2005

Table 2: tTA / rtTA responsive mouse lines

Genes under Tet Control
Reporter Genes	References
Fluorescent Calcium indicator Proteins: Cg2 (camdaroo 2); IP (inverse pericam); YC3.12 (yellow cameleon)	Hasan MT et al. 2004
Luciferase (L7) ¹⁾	Kistner A et al (1996)
lacZ	Mayford M et al (1996)
lacZ nls	Sarao R et al (1998)
GFP and lacZ	Krestel HE et al (2001)
Luciferase / Cre recombinase (LC-1)	Schonig K et al (2002)
CRE-IRES-tau/lacZ	Gogos JA et al (2002)
Genes of Interest	References
Adenylylcyclase type VI	Gao MH et al (2002)
AML1-ETO fusion	Rhoades KL et al (2000)
Ang-2 (Angiopoietin-2)	Oshima Y et al. (2005)
α AT (antitrypsin) wt and mutant forms	Hidvegi T et al. (2005)
Aurora-B and LacZ	Nguyen HG et al. (2005)
Axin	Hsu W et al (2001)
BCL6	Baron BW et al (2004)
BCR-ABL1 fusion	Huettner CS et al (2000)
BCR-ABL1 fusion	Koschmieder S et al (2005)
BMRRII (typeII receptor for bone morphogenetic peptides)	West J et al (2004)
BOB.1-OBF.1 /luc	Hess J et al (2001)
αCaMKII-Asp ²⁸⁶	Mayford M et al (1996)
Calcineurin autoinhibitory domain	Malleret G et al (2001)
Calcineurin (mutant)	Mansuy IM et al (1998)
cAMP response protein (CREB)	Chen J et al (1998)
cAMP response protein (CREB)	Sakai N et al (2002)
cAMP response protein (CREB)-VP16 fusion	Barco A et al (2002)
cAMP response protein (CREB) (mutant)	Pittenger C et al (2002)
Calpain inhibitor calpastatin	Grill MA et al (2003)
CD200	Yu K et al. (2005)
β-catenin / eGFP	Cheon SS et al (2002)
chemokine KC / lacZ	Wiekowski MT et al (2001)
colony stimulation factor-1 (CSF-1)	van Nguyen A et al (2002)
Cre recombinase	Saam JR et al (1999)
	Lindeberg J et al (2002)
	Radomska HS et al (2002)
	Perl AK et al (2002)
Luciferase / Cre recombinase (LC-1)	Schonig K et al (2002)
CRE-IRES-tau/lacZ	Gogos JA et al (2002)
CryAB r120G ( α -B-crystallin mutant)	Sanbe A et al. (2005)
CYP1B1	Hwang DY et al (2001)
Diphteria toxin A chain (DTA)	Lee P et al (1998)
Diphteria toxin A chain (mutant)	Gogos JA et al (2000)
Dystrophin	Ahmad A et al (2000)
Endostatin	Sund M et al. (2005)
Endothelin recptor B (EDNRB)	Shin M et al (1999)
epidermal growth factor receptor (EGFR)(truncated)	Roh M et al (2001)
ErbB2 receptor tyrosine kinase	Xie W et al (1999)
Estrogen receptor a	Hruska KS et al (2002)
Ferritin H - eGFP	Wilkinson J. et al. (2006)
FGF-receptor 2 (soluble dn mutant)	Hokuto I et al (2002)
fibroblast growth factor-7 (FGF 7)	Tichelaar JW et al (2000)
fibroblast growth factor-7 (hFGF 7)	Hayashi M et al (2005)
fibroblast growth factor-10 (FGF 10)	Clark JC et al (2001)
fibroblast growth factor-18 (FGF 18)	Whitsett JA et al (2002)
Flt3 ligand	Manfra DJ et al (2003)
Fokal adhesion kinase (FAK)	Peng Xu et al (2004)
forkhead transcription factor (FKHR) (trunctated)	Leenders H et al (2000)
ΔFosB ¹⁾	Chen J et al (1998)
FrzA (Frizzled-related protein) and LacZ	Baradon L et al. (2005)
acid a-glucosidase (GAA)	Raben N et al (2001)
hGCase (human acid ß-glucosidase)	Sun Y et al. (2006)
GATA 6	Liu C et al (2002)
GluR-A (eGFP-tagged) / lacZ	Mack V et al (2001)
Glycogen synthase kinase-3 β /lacZ	Lucas JJ et al (2001)
GR (rat)	Pazirandeh a et al. (2005)
Hepcidin	Viatte L. et al. (2006)
hormone sensitive lipase (HSL)	Suzuki J et al (2001)
Hox A10	Bjornsson JM et al (2001)
Huntingtin fragment and lacZ	Yamamoto A et al (2000)
Id1	Passman RS et al (1994)
Idx-1 hammerhead ribozyme	Thomas MK et al (2001)
IL-1ß (human)	Lappalainen U et al. (2005)
IL-10 (human)	Spight D et al. (2005)
α-integrin / luciferase	Valencik ML et al (2001)
Inverse pericam, camgaroo-2	Hasan MT et al (2004)
h-KCNQ2-G279 (domainant negative potassium channel mutant)	Peters HC et al. (2005)
Lymphocyte specific protein tyrosine kinase p56 ^lck	Legname G et al (2000)
M3	Pyo R et al (2004)
MHC class I H-2K ^b	Nagaraju K et al (2000)
MHC class II E α	Witherden D et al (2000)
Met receptor	Wang R et al (2001)
mineralocorticoid receptor antisense RNA	Beggah AT et al (2002)
c-myc	Felsher DW et al (1999)
	D´Cruz CM et al (2001)
	Marinkovic D et al (2004)
Nogo A / lacZ	Pot C et al (2002)
NRASV12	Wiesner SM et al (2005)
inducible NO-synthetase (iNOS) / eGFP	Mungrue IN et al (2002)
Nitric oxide synthase-2 (NOS-2)	Hjoberg J et al (2004)
NR1 / lacZ	Jerecic J et al (1999)
NR1 (N598R) / lacZ	Jerecic J et al (2001)
κ opiod receptor (RASSL) /lacZ ¹⁾	Redfern CS et al (1999)
Ornithine decarboxylase (ODC)	Guo Y et al (2000)
p25 (regulatory subunit of CdK5)	Cruz JC et al (2003)
p27 ^KIP1 / eGFP	Mitsuhashi T et al (2001)
parathyroid hormone related protein (PThrP)	Dunbar ME et al (2001)
Pdx1	Holland AM et al (2002)
Peripheral myelin protein 22 (PMP22)	Perea J et al (2001)
Prion (PrP ^C)	Tremblay P et al (1998)
Protein kinase C-β	Bowman JC et al (1997)
Protein kinase C-ε	Choi DS et al (2002)
γPKC-GFP	Sakai N et al (2004)
PKD2	Gallagher AR et al (2003)
Protein phosphatase 1	Genoux D et al (2002)
H-Ras ^V12G	Chin L et al (1999)
K-Ras ^G12D	Fisher GH et al (2001)
K-Ras ^G12D	Vitale-Cross L et al (2004)
Rb	Choi, YS et al. (2005)
RAR α	Yang Li et al (2003)
Retinoblastoma	Nikitin AY et al (2001)
SERCA 2a	Suarez J et al (2004)
Serotonin 1A receptor	Ghavami A et al (1999)
Serotonin 1A receptor	Gross C et al (2002)
Serotonin 1B receptor	Ghavami A et al (1999)
SK3	Taylor MS et al (2003)
SNARE, LacZ, and EGFP	Pascual O et al. (2005)
SOD (superoxide dismutase) / GPX (glutathione peroxidase)	Franke K et al (2004)
SOC S1 (JAB)	Petridou B et al (2003)
Stat 3C	Lian X et al. (2005)
surfactant protein B (SP-B)	Nesslein L et al. (2005)
surfactant protein D (SP-D)	Zhang L et al (2002)
SV40 large T antigen	Efrat S et al (1995)
	Manickan E et al (2001)
	Bendiksen S et al (2004)
T-cell receptor α OT1	LabrecqueN et al (2001)
TAC- β -integrin fusion / luciferase	Valencik ML et al (2001)
Tat	Kim BO et al (2003)
wt Tax, M22 Tax, M47 Tax	Kwon H et al. (2005)
TGFα (human)	Hardie WD et al. (2004)
TGF- β	Liu X et al (2001)
TGF- β	Ueberham E et al (2003)
TrK B	Ghersa P et al (1998)
TSLP (mouse) thymic stromal lymphopoietin	Yoo J et al. 2005
Tyrosinase	Gimenez E et al (2004)
urokinase-like plasmidogen activator (uPA)	Sisson TH et al (2002)
utropin	Squire S et al (2002)
vascular chymase (RVCH)	Ju H et al (2001)
vascular endothelial growth factor (VEGF)	Ohno-Matsui K et al (2002)
	Dor Y et al (2001)
	Baluk P et al (2004)
	Lee CG et al. (2004)
Wnt1	Gunther EJ et al (2003)

nls = nuclear localization signal

1) mouse line available at ‘The Jackson Laboratory’, Bar Harbor, ME 04609, USA http://www.jax.org/